噬菌体RZⅢ-3的生物学特性分析及对鸡大肠杆菌的疗效

doi:10.11843/j.issn.0366-6964.2017.11.018

Abstract

Abstract:

To solve the antibiotic resistance and find substitution of antibiotics, this study explored the basic biological characteristics of Escherichia coli phage RZⅢ-3 and its therapeutic effect on SPF chicken colibacillosis. The biological characteristics of Escherichia coli phage RZⅢ-3 showed that the titer was 10⁹ pfu·mL^-1, high stability to heat, the bacteriophage titer can reach more than 10⁷ pfu·mL^-1 after incubation at 60℃ for 1 h. It had a wide adaptive range of pH, and maintained a high cleavage activity at pH 4-10. The optimum pH of the phage was about 7.0, its optimal multiplicity of infection (MOI) was 0.1, and the incubation period of infected host bacteria was about 20 min. The results of transmission electron microscopy showed that the phage belonged to the T4 phage. Escherichia coli phage RZⅢ-3 on Escherichia coli 1019 had a strong ability to crack, The number of Escherichia coli 1019 in SPF chickens can be reduced by 96.5%.This indicated that the Escherichia coli phage RZⅢ-3 has a greater potential for the treatment of specific Escherichia coli infection.

CLC Number:

Q939.48

WANG Jie, LI Lu-lu, ZHANG Qing. Biological Characteristics Analysis of Phage RZⅢ-3 to Escherichia coli and the Therapeutic Effect on Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2166-2172.

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Biological Characteristics Analysis of Phage RZⅢ-3 to Escherichia coli and the Therapeutic Effect on Chicken

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